ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
최상의 결과를 위해서는 올바른 시약을 사용함으로써 피크 대칭성을 개선할 수 있습니다.
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物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
The information acquisition system data and analyses the detector indicators, enabling chemical substances to become quantified dependent on their peak regions from the chromatogram.
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-hydroxybenzoic acid (PH) over a nonpolar C18 column subject to some optimum analysis time of 6 min. The shaded regions stand for regions exactly where a separation is not possible, With all the unresolved solutes identified.
. One particular problem by having an isocratic elution is an correct cell period check here energy for resolving early-eluting solutes here may perhaps bring about unacceptably lengthy retention times for late-eluting solutes. Optimizing the cell period for late-eluting solutes, Alternatively, may perhaps offer an inadequate separation of early-eluting solutes.
The quick and productive setting up of the column will take years to grasp. Here are several strategies and methods to setup the right column
Broadened peaks can obscure concentrate on peaks and make quantification tricky. Here are a few popular results in and methods for peak broadening:
The column will be the separation chamber where by the magic of HPLC transpires. It properties the stationary stage, a packed bed of microscopic particles.
Widespread mobile section modifiers like acids and bases might be added to good-tune the conversation between analytes plus the column. These modifiers can:
특히 컬럼의 선정은 분석의 결과에 영향을 미치기에 신중하게 선택하여야 합니다.
The liquid that transports the sample with the column is known as the mobile stage. It comprises of a number of solvents selected dependant on the analysis’s special demands.